June 15, 1998From John Dvorak:
You can search the entire medline database for free by following this link http://www.nlm.nih.gov/databases/freemedl.html
This database has a tremendous amount of information. By using keywords such as
cannabis, hemp, and marijuana, youll find the references to thousands of published
articles. Many of the references include an abstract which gives additional info.
The first item below is a new study that I just heard about thanks to Rob Clarke of the
International Hemp Association and Remy Chevalier. I think it could be important.
Happy searching and bast regards.
John Dvorak

Medline search for "cannabis" and "amino":
The Journal of Bioscience, Biotechnology, and Biochemistry
Biosci Biotechnol Biochem 1998 Apr;62(4):650-654
Isolation and primary structure of a methionine- and cystine-rich seed Protein of
Cannabis sativa.
Odani S, Odani S
Department of Home Economics, Faculty of Education, Niigata University, Japan.
Abstract:
A 10-kDa protein was isolated from resting seeds of hemp (Cannabis sativa) by buffer
extraction, gel filtration, ion-exchange chromatography, and reversed-phase high-pressure
liquid chromatography. The protein did not inhibit bovine trypsin. It consisted of
subunits composed of 27 and 61 residues and was held together by two disulfide bonds. The
complete amino acid sequence was identified by protein analysis, and had 20 mole% of amino
acids containing sulfur. The protein was most similar to a methionine-rich protein of
Brazil nut (Bertholletia excelsa) and to Mabinlin IV, a sweetness-inducing protein of
Capparis masaikai. The high methionine content and the absence of
trypsin inhibitory activity suggested that the seed protein can be used to improve the
nutritional quality of plant food-stuffs.
PMID: 9614695, UI: 98276879

A search for "hemp" "seed" and "oil" resulted in these
hits:
Lehmann T, et al.
Excretion of cannabinoids in urine after ingestion of cannabis seed oil.
J Anal Toxicol. 1997 Sep; 21(5): 373-375.
PMID: 9288590; UI: 97434722.

Callaway JC, et al.
A positive THC urinalysis from hemp (Cannabis) seed oil.
J Anal Toxicol. 1997 Jul; 21(4): 319-320. No abstract available.
PMID: 9248953; UI: 97392220.

Struempler RE, et al.
A positive cannabinoids workplace drug test following the ingestion of commercially
available hemp seed oil.
J Anal Toxicol. 1997 Jul; 21(4): 283-285.
PMID: 9248945; UI: 97392212.

Meier H, et al.
[Cannabis poisoning after eating salad].
Schweiz Med Wochenschr. 1997 Feb 8; 127(6): 214-218.
[Article in German]
Meier H, Vonesch HJ
Medizinische Abteilung Bezirksspital Zofingen.
We describe 4 patients who suffered gastrointestinal disorders and psychological
effects after eating salad prepared with hemp seed oil. The
concentration of tetrahydrocannabinol (THC) in this oil far exceeded the recommended
tolerance dose. Our observations prompted the Swiss Federal Office of Public Health to
publish warnings in the press concerning consumption of this oil. We describe the
symptoms of orally ingested THC and point out unresolved problems connected with food
containing hemp.
PMID: 9157527, UI: 97208122

Fryday SL, et al.
Effects of exposure to an organophosphate on the seed-handling efficiency of the house
sparrow.
Bull Environ Contam Toxicol. 1994 Dec; 53(6): 869-876. No abstract available.
PMID: 7881216; UI: 95186926.

Cortis G, et al.
Experimental cultivation of cannabis plants in the Mediterranean area.
Bull Narc. 1985 Oct; 37(4): 67-73.
PMID: 3011161; UI: 86216786.

Jacks TJ, et al.
Conformations of oilseed storage proteins (globulins) determined by circular dichroism.
Int J Pept Protein Res. 1973; 5(4): 289-291. No abstract available.
PMID: 4796700; UI: 74046684.

Vieira JE, et al.
On the pharmacology of the hemp seed oil.
Med Pharmacol Exp Int J Exp Med. 1967; 16(3): 219-224. No abstract available.
PMID: 6071763; UI: 67201203.

The Hemp Page of Marijuananews.com is edited
by John E. Dvorak, Hempologist &
Managing Editor, Hemp Magazine.
John was born in Fort Worth, Texas, but is an eight year resident
of Allston/Brighton, MA, where he is the proprietor of the Boston Hemp Co-op and Managing
Editor of Hemp Magazine. He is a member of the Hemp Industries Association, the
International Hemp Association, and Mass/Cann NORML.
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